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a-d: <t>2D</t> <t>neuronal</t> cultures; e-i: 3D-hCSA; j-m: human brain. (a) Representative confocal images of isogenic 2D neurons and the <t>same</t> <t>Imaris</t> <t>9.9.1</t> processed images. Different colour of neurites are Imaris software coded and represents diameter, length and branches. Scale bar: 10 µm. (b) T21 dendrites showed larger diameter, (c) dendrites were shorter with (d) fewer branches compared to the D21 dendrites. (e) Representative confocal images of isogenic hCSAs neurons. Scale bar: 10 µm. (f) Representative TEM images of isogenic dendrites, magnification 20,000x. The diameter of the T21 dendrite was 3.0 µm, whereas the D21 dendrite was 2.6 µm. (g) T21 dendrites showed larger diameter, (h) dendrites were shorter with (i) fewer branches compared to the D21 dendrites. (j) Representative confocal images of cortical neurons from human brains. (k) T21 dendrites showed larger diameter, (l) dendrites were shorter with (m) fewer branches compared to the D21 dendrites. Graphs represent means ± SEM.
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a-d: 2D neuronal cultures; e-i: 3D-hCSA; j-m: human brain. (a) Representative confocal images of isogenic 2D neurons and the <t>same</t> <t>Imaris</t> <t>9.9.1</t> processed images. Different colour of neurites are Imaris software coded and represents diameter, length and branches. Scale bar: 10 µm. (b) T21 dendrites showed larger diameter, (c) dendrites were shorter with (d) fewer branches compared to the D21 dendrites. (e) Representative confocal images of isogenic hCSAs neurons. Scale bar: 10 µm. (f) Representative TEM images of isogenic dendrites, magnification 20,000x. The diameter of the T21 dendrite was 3.0 µm, whereas the D21 dendrite was 2.6 µm. (g) T21 dendrites showed larger diameter, (h) dendrites were shorter with (i) fewer branches compared to the D21 dendrites. (j) Representative confocal images of cortical neurons from human brains. (k) T21 dendrites showed larger diameter, (l) dendrites were shorter with (m) fewer branches compared to the D21 dendrites. Graphs represent means ± SEM.
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a-d: 2D neuronal cultures; e-i: 3D-hCSA; j-m: human brain. (a) Representative confocal images of isogenic 2D neurons and the same Imaris 9.9.1 processed images. Different colour of neurites are Imaris software coded and represents diameter, length and branches. Scale bar: 10 µm. (b) T21 dendrites showed larger diameter, (c) dendrites were shorter with (d) fewer branches compared to the D21 dendrites. (e) Representative confocal images of isogenic hCSAs neurons. Scale bar: 10 µm. (f) Representative TEM images of isogenic dendrites, magnification 20,000x. The diameter of the T21 dendrite was 3.0 µm, whereas the D21 dendrite was 2.6 µm. (g) T21 dendrites showed larger diameter, (h) dendrites were shorter with (i) fewer branches compared to the D21 dendrites. (j) Representative confocal images of cortical neurons from human brains. (k) T21 dendrites showed larger diameter, (l) dendrites were shorter with (m) fewer branches compared to the D21 dendrites. Graphs represent means ± SEM.

Journal: bioRxiv

Article Title: Abnormal neuronal and synaptic morphology in Down syndrome brains reproduces in 2D and 3D human cellular models

doi: 10.64898/2026.01.10.698781

Figure Lengend Snippet: a-d: 2D neuronal cultures; e-i: 3D-hCSA; j-m: human brain. (a) Representative confocal images of isogenic 2D neurons and the same Imaris 9.9.1 processed images. Different colour of neurites are Imaris software coded and represents diameter, length and branches. Scale bar: 10 µm. (b) T21 dendrites showed larger diameter, (c) dendrites were shorter with (d) fewer branches compared to the D21 dendrites. (e) Representative confocal images of isogenic hCSAs neurons. Scale bar: 10 µm. (f) Representative TEM images of isogenic dendrites, magnification 20,000x. The diameter of the T21 dendrite was 3.0 µm, whereas the D21 dendrite was 2.6 µm. (g) T21 dendrites showed larger diameter, (h) dendrites were shorter with (i) fewer branches compared to the D21 dendrites. (j) Representative confocal images of cortical neurons from human brains. (k) T21 dendrites showed larger diameter, (l) dendrites were shorter with (m) fewer branches compared to the D21 dendrites. Graphs represent means ± SEM.

Article Snippet: In order to describe and analyse observed neuronal morphology quantitative analysis of confocal images was performed by Imaris 9.9.1 software.

Techniques: Software

a-d: 2D neuronal cultures; e-i: 3D-hCSA; j-m: human brain. (a) Representative confocal images of isogenic 2D neurons and the same Imaris 9.9.1 processed images. Different colour of neurites are Imaris software coded and represents diameter, length and branches. Scale bar: 10 µm. (b) T21 dendrites showed larger diameter, (c) dendrites were shorter with (d) fewer branches compared to the D21 dendrites. (e) Representative confocal images of isogenic hCSAs neurons. Scale bar: 10 µm. (f) Representative TEM images of isogenic dendrites, magnification 20,000x. The diameter of the T21 dendrite was 3.0 µm, whereas the D21 dendrite was 2.6 µm. (g) T21 dendrites showed larger diameter, (h) dendrites were shorter with (i) fewer branches compared to the D21 dendrites. (j) Representative confocal images of cortical neurons from human brains. (k) T21 dendrites showed larger diameter, (l) dendrites were shorter with (m) fewer branches compared to the D21 dendrites. Graphs represent means ± SEM.

Journal: bioRxiv

Article Title: Abnormal neuronal and synaptic morphology in Down syndrome brains reproduces in 2D and 3D human cellular models

doi: 10.64898/2026.01.10.698781

Figure Lengend Snippet: a-d: 2D neuronal cultures; e-i: 3D-hCSA; j-m: human brain. (a) Representative confocal images of isogenic 2D neurons and the same Imaris 9.9.1 processed images. Different colour of neurites are Imaris software coded and represents diameter, length and branches. Scale bar: 10 µm. (b) T21 dendrites showed larger diameter, (c) dendrites were shorter with (d) fewer branches compared to the D21 dendrites. (e) Representative confocal images of isogenic hCSAs neurons. Scale bar: 10 µm. (f) Representative TEM images of isogenic dendrites, magnification 20,000x. The diameter of the T21 dendrite was 3.0 µm, whereas the D21 dendrite was 2.6 µm. (g) T21 dendrites showed larger diameter, (h) dendrites were shorter with (i) fewer branches compared to the D21 dendrites. (j) Representative confocal images of cortical neurons from human brains. (k) T21 dendrites showed larger diameter, (l) dendrites were shorter with (m) fewer branches compared to the D21 dendrites. Graphs represent means ± SEM.

Article Snippet: Confocal images were captured by Olympus FV 3000 microscope and further analysed using Imaris 9.9.1 - Neuroscience module software (BITPLANE, An Oxford Instruments Co., Zurich, Switzerland).

Techniques: Software